Somatic cells can be reprogrammed into totipotent or pluripotent state by nuclear transfer (NT) or the ‘Yamanaka method’. White adipose tissue (WAT) is a source of readily accessible donor cells that provide virtually unlimited cells for reprogramming. Recently, freshly isolated white adipose tissue-derived cells (ADCs) from humans or mice have been successfully reprogrammed into induced pluripotent stem cells (iPSCs) by ‘Yamanaka factors’ (Sun et al., 2009; Sugii et al., 2010). In contrast, cultured mouse ADCs were not feasible donors for NT, most likely due to chromosome instability that might occur during in vitro culture (Qin et al., 2009). Therefore, it remains unknown whether genomic stability sustains in freshly isolated mouse ADCs that can be used as donors for NT. Here, we report that cloned mice can be produced from ADCs and cloning efficiency can be dramatically improved by replacing the trophoblast cells in the cloned blastocysts with cells from tetraploid embryos. Moreover, the cloning efficiencies are consistently higher for Lin⁻ cells than for CD45⁺ cells. Our results indicate that ADCs are an easily obtainable cell source that can be used as donors for studying somatic reprogramming induced by oocytes.